Protease Inhibitor:Article Title: MST1R-targeted therapy in the battle against gallbladder cancer.
Article Snippet: .. GBC-SD cells were exposed to varying concentrations of MGCD-265 (#S1361, Selleck) for 24 h. RIPA lysate (#PC101, Epizyme Biotech, Shanghai, China), supplemented with Protease Inhibitor Cocktail (#P8340, SigmaAldrich LLC), was employed for cell lysis, followed by a 30-minute incubation on ice and subsequent centrifugation at 4 °C for 15 min at 12,000 g. The resulting supernatant was collected and assessed for protein concentration using the BCA method (#P0012, Beyotime Biotechnology, China). .. Gel electrophoresis was executed utilizing an SDS-PAGE separator gel (#PG112 and #PG113, Epizyme Biotech, Shanghai, China), with each sample loaded containing an equal protein quantity of 30 μg.
Article Title: MST1R-targeted therapy in the battle against gallbladder cancer
Article Snippet: .. GBC-SD cells were exposed to varying concentrations of MGCD-265 (#S1361, Selleck) for 24 h. RIPA lysate (#PC101, Epizyme Biotech, Shanghai, China), supplemented with Protease Inhibitor Cocktail (#P8340, Sigma-Aldrich LLC), was employed for cell lysis, followed by a 30-minute incubation on ice and subsequent centrifugation at 4 °C for 15 min at 12,000 g. The resulting supernatant was collected and assessed for protein concentration using the BCA method (#P0012, Beyotime Biotechnology, China). .. Gel electrophoresis was executed utilizing an SDS-PAGE separator gel (#PG112 and #PG113, Epizyme Biotech, Shanghai, China), with each sample loaded containing an equal protein quantity of 30 μg.
Lysis:Article Title: MST1R-targeted therapy in the battle against gallbladder cancer.
Article Snippet: .. GBC-SD cells were exposed to varying concentrations of MGCD-265 (#S1361, Selleck) for 24 h. RIPA lysate (#PC101, Epizyme Biotech, Shanghai, China), supplemented with Protease Inhibitor Cocktail (#P8340, SigmaAldrich LLC), was employed for cell lysis, followed by a 30-minute incubation on ice and subsequent centrifugation at 4 °C for 15 min at 12,000 g. The resulting supernatant was collected and assessed for protein concentration using the BCA method (#P0012, Beyotime Biotechnology, China). .. Gel electrophoresis was executed utilizing an SDS-PAGE separator gel (#PG112 and #PG113, Epizyme Biotech, Shanghai, China), with each sample loaded containing an equal protein quantity of 30 μg.
Article Title: MST1R-targeted therapy in the battle against gallbladder cancer
Article Snippet: .. GBC-SD cells were exposed to varying concentrations of MGCD-265 (#S1361, Selleck) for 24 h. RIPA lysate (#PC101, Epizyme Biotech, Shanghai, China), supplemented with Protease Inhibitor Cocktail (#P8340, Sigma-Aldrich LLC), was employed for cell lysis, followed by a 30-minute incubation on ice and subsequent centrifugation at 4 °C for 15 min at 12,000 g. The resulting supernatant was collected and assessed for protein concentration using the BCA method (#P0012, Beyotime Biotechnology, China). .. Gel electrophoresis was executed utilizing an SDS-PAGE separator gel (#PG112 and #PG113, Epizyme Biotech, Shanghai, China), with each sample loaded containing an equal protein quantity of 30 μg.
Incubation:Article Title: MST1R-targeted therapy in the battle against gallbladder cancer.
Article Snippet: .. GBC-SD cells were exposed to varying concentrations of MGCD-265 (#S1361, Selleck) for 24 h. RIPA lysate (#PC101, Epizyme Biotech, Shanghai, China), supplemented with Protease Inhibitor Cocktail (#P8340, SigmaAldrich LLC), was employed for cell lysis, followed by a 30-minute incubation on ice and subsequent centrifugation at 4 °C for 15 min at 12,000 g. The resulting supernatant was collected and assessed for protein concentration using the BCA method (#P0012, Beyotime Biotechnology, China). .. Gel electrophoresis was executed utilizing an SDS-PAGE separator gel (#PG112 and #PG113, Epizyme Biotech, Shanghai, China), with each sample loaded containing an equal protein quantity of 30 μg.
Article Title: MST1R-targeted therapy in the battle against gallbladder cancer
Article Snippet: .. GBC-SD cells were exposed to varying concentrations of MGCD-265 (#S1361, Selleck) for 24 h. RIPA lysate (#PC101, Epizyme Biotech, Shanghai, China), supplemented with Protease Inhibitor Cocktail (#P8340, Sigma-Aldrich LLC), was employed for cell lysis, followed by a 30-minute incubation on ice and subsequent centrifugation at 4 °C for 15 min at 12,000 g. The resulting supernatant was collected and assessed for protein concentration using the BCA method (#P0012, Beyotime Biotechnology, China). .. Gel electrophoresis was executed utilizing an SDS-PAGE separator gel (#PG112 and #PG113, Epizyme Biotech, Shanghai, China), with each sample loaded containing an equal protein quantity of 30 μg.
Centrifugation:Article Title: MST1R-targeted therapy in the battle against gallbladder cancer.
Article Snippet: .. GBC-SD cells were exposed to varying concentrations of MGCD-265 (#S1361, Selleck) for 24 h. RIPA lysate (#PC101, Epizyme Biotech, Shanghai, China), supplemented with Protease Inhibitor Cocktail (#P8340, SigmaAldrich LLC), was employed for cell lysis, followed by a 30-minute incubation on ice and subsequent centrifugation at 4 °C for 15 min at 12,000 g. The resulting supernatant was collected and assessed for protein concentration using the BCA method (#P0012, Beyotime Biotechnology, China). .. Gel electrophoresis was executed utilizing an SDS-PAGE separator gel (#PG112 and #PG113, Epizyme Biotech, Shanghai, China), with each sample loaded containing an equal protein quantity of 30 μg.
Article Title: MST1R-targeted therapy in the battle against gallbladder cancer
Article Snippet: .. GBC-SD cells were exposed to varying concentrations of MGCD-265 (#S1361, Selleck) for 24 h. RIPA lysate (#PC101, Epizyme Biotech, Shanghai, China), supplemented with Protease Inhibitor Cocktail (#P8340, Sigma-Aldrich LLC), was employed for cell lysis, followed by a 30-minute incubation on ice and subsequent centrifugation at 4 °C for 15 min at 12,000 g. The resulting supernatant was collected and assessed for protein concentration using the BCA method (#P0012, Beyotime Biotechnology, China). .. Gel electrophoresis was executed utilizing an SDS-PAGE separator gel (#PG112 and #PG113, Epizyme Biotech, Shanghai, China), with each sample loaded containing an equal protein quantity of 30 μg.
Protein Concentration:Article Title: MST1R-targeted therapy in the battle against gallbladder cancer.
Article Snippet: .. GBC-SD cells were exposed to varying concentrations of MGCD-265 (#S1361, Selleck) for 24 h. RIPA lysate (#PC101, Epizyme Biotech, Shanghai, China), supplemented with Protease Inhibitor Cocktail (#P8340, SigmaAldrich LLC), was employed for cell lysis, followed by a 30-minute incubation on ice and subsequent centrifugation at 4 °C for 15 min at 12,000 g. The resulting supernatant was collected and assessed for protein concentration using the BCA method (#P0012, Beyotime Biotechnology, China). .. Gel electrophoresis was executed utilizing an SDS-PAGE separator gel (#PG112 and #PG113, Epizyme Biotech, Shanghai, China), with each sample loaded containing an equal protein quantity of 30 μg.
Article Title: MST1R-targeted therapy in the battle against gallbladder cancer
Article Snippet: .. GBC-SD cells were exposed to varying concentrations of MGCD-265 (#S1361, Selleck) for 24 h. RIPA lysate (#PC101, Epizyme Biotech, Shanghai, China), supplemented with Protease Inhibitor Cocktail (#P8340, Sigma-Aldrich LLC), was employed for cell lysis, followed by a 30-minute incubation on ice and subsequent centrifugation at 4 °C for 15 min at 12,000 g. The resulting supernatant was collected and assessed for protein concentration using the BCA method (#P0012, Beyotime Biotechnology, China). .. Gel electrophoresis was executed utilizing an SDS-PAGE separator gel (#PG112 and #PG113, Epizyme Biotech, Shanghai, China), with each sample loaded containing an equal protein quantity of 30 μg.
|
Selleck Chemicals is a verified supplier 
Selleck Chemicals manufactures this product 